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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Magnetic Resonance Imaging01:24

Magnetic Resonance Imaging

Magnetic resonance imaging (MRI) is a noninvasive medical imaging technique based on a phenomenon of nuclear physics discovered in the 1930s, in which matter exposed to magnetic fields and radio waves was found to emit radio signals. In 1970, a physician and researcher named Raymond Damadian noticed that malignant (cancerous) tissue gave off different signals than normal body tissue. He applied for a patent for the first MRI scanning device in clinical use by the early 1980s. The early MRI...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

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Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities
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Metadata matters: access to image data in the real world.

Melissa Linkert1, Curtis T Rueden, Chris Allan

  • 1Laboratory for Optical and Computational Instrumentation, Department of Molecular Biology, Graduate School, University of Wisconsin at Madison, Madison, WI 53711, USA.

The Journal of Cell Biology
|June 2, 2010
PubMed
Summary
This summary is machine-generated.

To improve biological data sharing, this study introduces a new open standard format for microscopy images. This format aims to overcome proprietary file format challenges and support data repositories.

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Area of Science:

  • Microscopy
  • Biological Sciences
  • Data Science

Background:

  • Data sharing is crucial for scientific integrity and discovery in biological sciences.
  • Multidimensional microscopy image data present challenges due to diverse proprietary file formats (PFFs).
  • Centralized repositories and common data formats are essential for effective data sharing.

Purpose of the Study:

  • To develop and propose an open standard format for microscopy image data.
  • To address the limitations imposed by proprietary file formats in scientific image data management.
  • To advocate for community adoption of open image data standards.

Main Methods:

  • Development of a novel open standard format specifically for microscopy image data.
  • Analysis of existing proprietary file formats (PFFs) to identify interoperability challenges.
  • Community engagement to promote the adoption of the proposed open standard.

Main Results:

  • A new open standard format for multidimensional microscopy image data has been successfully developed.
  • The proposed format facilitates data sharing and interoperability across different imaging platforms.
  • The development lays the groundwork for a centralized, open image data repository.

Conclusions:

  • Adoption of open image data standards is essential for advancing biological research.
  • Standardized formats will enhance the utility and accessibility of microscopy image data.
  • The developed open standard format provides a foundation for future open data repositories.