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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

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Related Experiment Video

Updated: Jun 12, 2026

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
09:12

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities

Published on: April 22, 2013

A multipurpose modular system for high-resolution microscopy at high hydrostatic pressure.

Hugh Vass1, S Lucas Black, Eva M Herzig

  • 1SUPA, School of Physics and Astronomy, University of Edinburgh, James Clerk Maxwell Building, The King's Buildings, Mayfield Road, Edinburgh EH9 3JZ, United Kingdom.

The Review of Scientific Instruments
|June 3, 2010
PubMed
Summary
This summary is machine-generated.

We created a versatile high-pressure microscopy system for studying materials and biological samples. This modular setup enables high-resolution imaging under diverse pressure and temperature conditions, revealing phase transitions and bacterial growth.

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Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
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Area of Science:

  • Biophysics
  • Materials Science
  • Microscopy

Background:

  • High hydrostatic pressure microscopy is crucial for understanding cellular processes and material phase transitions.
  • Existing systems often lack modularity and precise control over pressure and temperature.

Purpose of the Study:

  • To develop and demonstrate a modular, high-resolution microscopy system capable of operating at high hydrostatic pressures.
  • To showcase the system's versatility for various scientific applications, including fluid dynamics and microbiology.

Main Methods:

  • Designed a modular system with interchangeable components: a pressurized cell (approx. 100 microl), temperature-controlled holder, ram, and piston.
  • Integrated confocal/epifluorescence and transmitted light microscopy capabilities.
  • Utilized pentyl-cyanobiphenyl (5CB) liquid crystal phase transition for pressure calibration.

Main Results:

  • Developed pressure cells with ranges of 0.1-700 MPa and 0.1-100 MPa.
  • Demonstrated time-resolved imaging of colloidal phase transitions (0.1-100 MPa) and *Escherichia coli* growth (50 MPa).
  • Established 5CB liquid crystal phase transition as a reliable pressure calibration method (0.1-200 MPa).

Conclusions:

  • The modular high-pressure microscopy system offers precise control over pressure and temperature (-20 to 70 °C) with rapid quenching capabilities.
  • The system is suitable for diverse applications, including studying colloidal systems and microbial growth under pressure.
  • Validated the system's performance and utility through multiple experimental demonstrations and a novel calibration technique.