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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Halo columns: new generation technology for high speed liquid chromatography
Imran Ali1, Vinay D Gaitonde, Anders Grahn
1Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi-110025, India. drimran_ali@yahoo.com
Journal of Chromatographic Science
|June 3, 2010
Summary
Superficially porous silica particle columns offer fast speed and high sample loading, meeting industry demands. Halo columns, a key example, provide efficient separation for various compounds.
Area of Science:
- Analytical Chemistry
- Chromatography Science
Background:
- Industry and research demand faster analytical methods and higher sample throughput.
- Conventional chromatography columns face limitations in speed and efficiency.
- Superficially porous silica particle columns have emerged as a promising alternative.
Purpose of the Study:
- To highlight the importance of superficially porous silica particle columns.
- To provide an in-depth look at Halo columns, a leading product in this category.
- To discuss the structure, separation mechanisms, applications, and comparative performance of these advanced columns.
Main Methods:
- Review and discussion of the structural characteristics of superficially porous silica particle columns.
- Analysis of separation mechanisms, particularly focusing on Halo columns.
- Comparative assessment against traditional packed-bed chromatography columns.
Main Results:
- Superficially porous columns exhibit superior speed, sharper peaks, and good sample loading capacity.
- Halo columns, with specific dimensions (2.7 µm OD, 0.5 µm porous layer, 90 Å pores, 150 m²/g surface area), are effective for low molecular weight compounds.
- These columns demonstrate significant advantages over conventional column technologies.
Conclusions:
- Superficially porous silica particle columns, especially Halo columns, represent a significant advancement in chromatography.
- Their unique structure enables faster separations and improved efficiency for a range of analytes.
- These columns are crucial for meeting the evolving demands of modern analytical science.
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