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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Updated: Jun 12, 2026

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
14:49

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Published on: October 27, 2011

A novel method for constructing pathogen-regulated small RNA cDNA library.

Chuan Bao Sun1, Xian Ming Du, Yu Ke He

  • 1National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. cbsun@sibs.ac.cn

Biochemical and Biophysical Research Communications
|June 3, 2010
PubMed
Summary
This summary is machine-generated.

Researchers identified small RNAs in Brassica campestris (Bcp) plants responding to Erwinia carotovora subsp. carotovora (Ecc) infection. This discovery aids understanding of plant immune responses and soft rot disease regulation.

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A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
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A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

Published on: December 6, 2017

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Last Updated: Jun 12, 2026

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA
14:49

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Published on: October 27, 2011

A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
10:32

A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

Published on: December 6, 2017

Area of Science:

  • Plant molecular biology
  • RNA biology
  • Plant pathology

Background:

  • Endogenous small non-coding RNAs are crucial for plant immunity via RNA silencing.
  • Erwinia carotovora subsp. carotovora (Ecc) causes soft rot disease in Brassica campestris (Bcp).

Purpose of the Study:

  • To discover endogenous small RNA species in Bcp that are regulated by Ecc infection.
  • To develop an efficient method for cloning pathogen-regulated small RNAs.

Main Methods:

  • Designed degenerate stem-loop reverse primers for cDNA synthesis.
  • Tailed cDNA with poly(C) for forward priming.
  • Employed cDNA/RNA subtractive hybridization to capture Ecc-regulated small RNAs.
  • Constructed small RNA cDNA libraries for sequencing.

Main Results:

  • Successfully developed a method to clone pathogen-regulated small RNAs.
  • Identified specific small RNA species in Bcp in response to Ecc infection (sequencing data not detailed in abstract).

Conclusions:

  • The developed approach is effective for identifying pathogen-responsive small RNAs.
  • This research provides insights into the molecular mechanisms of plant defense against bacterial pathogens.