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Updated: Jun 12, 2026
06:48
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
Published on: June 16, 2022
Emerging paradigms of regulated microRNA processing.
Martin A Newman1, Scott M Hammond
1Department of Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Genes & Development
|June 3, 2010
Summary
This review explores how microRNAs (miRNAs) are regulated post-transcriptionally. It highlights two key mechanisms involving the Microprocessor complex and RNA-binding proteins controlling miRNA biogenesis and gene expression.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- MicroRNAs (miRNAs) are crucial regulators of gene expression impacting development, tissue homeostasis, and disease pathogenesis.
- Precise control over miRNA levels is vital and achieved through intricate regulation of the miRNA biogenesis pathway.
Purpose of the Study:
- To review emerging paradigms in post-transcriptional regulation of microRNA expression.
- To elucidate the mechanisms controlling miRNA abundance and function.
Main Methods:
- Literature review focusing on post-transcriptional regulatory mechanisms of miRNA biogenesis.
- Analysis of the roles of the Microprocessor complex and RNA-binding proteins in miRNA maturation.
Main Results:
- Two primary paradigms of post-transcriptional miRNA control are identified.
- The Microprocessor complex is essential for canonical miRNA maturation.
- A second paradigm involves RNA-binding proteins and cis-regulatory sequences in precursor miRNA loops, particularly for miRNA families.
Conclusions:
- Post-transcriptional regulation significantly impacts miRNA abundance and function.
- Understanding these regulatory mechanisms is key to comprehending gene expression control in health and disease.
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