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Updated: Jun 12, 2026

09:27
Protein Crystallization for X-ray Crystallography
Published on: January 16, 2011
Amino acids and glycine ethyl ester as new crystallization reagents for lysozyme
Len Ito1, Kentaro Shiraki, Hiroshi Yamaguchi
1Japan Synchrotron Radiation Research Institute (SPring-8), Japan. l-ito@spring8.or.jp
Abstract:
Several amino acids and their derivatives are prominent additives in the field of protein chemistry. This study reports the use of charged amino acids and glycine ethyl ester as precipitants in protein crystallization, using hen egg-white lysozyme (HEWL) as a model. A discussion of the crystallization of HEWL using these reagents as precipitating agents is given.

