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Cytokines in fluids from polycystic kidneys.
K D Gardner1, J S Burnside, L W Elzinga
1Department of Medicine, University of New Mexico School of Medicine, Albuquerque.
Kidney International
|April 1, 1991
Summary
Cytokines, including interleukin-1 beta, are present in autosomal dominant polycystic kidney disease (ADPKD) cyst fluids. These findings suggest cytokines contribute to ADPKD pathogenesis and related complications.
Area of Science:
- Nephrology
- Immunology
- Molecular Biology
Background:
- Autosomal dominant polycystic kidney disease (ADPKD) is a genetic disorder characterized by cyst formation in the kidneys.
- The pathogenesis of ADPKD involves complex molecular and cellular processes, including inflammation.
Purpose of the Study:
- To investigate the presence and bioactivity of cytokines in cyst fluids from ADPKD patients.
- To explore the potential role of cytokines in the morbidity and pathogenesis of ADPKD.
Main Methods:
- Analysis of 104 aerobic culture-negative cyst fluids from 13 ADPKD patients.
- Detection of interleukin-1 beta (IL-1 beta), interleukin-2 (IL-2), tumor necrosis factor alpha (TNF alpha), stromelysin, and prostaglandin E2 (PGE2) using ELISAs and radioimmunoassay.
- Assessment of IL-1 beta bioactivity through thymocyte proliferation assays.
Main Results:
- Interleukin-1 beta (IL-1 beta) was detected in 65% of cyst fluids, with significant bioactivity observed in 36 out of 51 fluids.
- Elevated concentrations of IL-1 beta correlated with increased levels of IL-2, TNF alpha, stromelysin, and PGE2, suggesting in vivo cytokine cascade activation.
- Cytokine concentrations did not correlate with fluid osmolality, solute concentration, or endotoxin activity.
Conclusions:
- Cytokines, particularly IL-1 beta, are present and bioactive in ADPKD cyst fluids.
- These findings identify cytokines as potential contributors to ADPKD morbidity and pathogenesis.
- Further research into cytokine-targeted therapies for ADPKD is warranted.