Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Application of Multidimensional Covalent Organic Frameworks for Enhanced CO<sub>2</sub> Adsorption.

Chemical record (New York, N.Y.)·2026
Same author

Advancing Nutritional Status Classification With Hybrid Artificial Intelligence: A Novel Methodological Approach.

Brain and behavior·2025
Same author

Systematic review of differentially abundant proteins in people with Lewy body dementia.

Acta neuropsychiatrica·2025
Same author

Unveiling cutting-edge developments: architectures and nanostructured materials for application in optoelectronic artificial synapses.

Nanoscale·2024
Same author

Cobalt-doped zinc oxide based memristors with nociceptor characteristics for bio-inspired technology.

RSC advances·2024
Same author

Density Functional Theory Unveils the Secrets of SiAuF<sub>3</sub> and SiCuF<sub>3</sub>: Exploring Their Striking Structural, Electronic, Elastic, and Optical Properties.

Molecules (Basel, Switzerland)·2024

Related Experiment Video

Updated: Jun 12, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Geometric superresolution by using an optical mask.

Mohammad Sohail1, Asloob A Mudassar

  • 1Department of Physics and Applied Mathematics, Pakistan Institute of Engineering and Applied Sciences, 45650 Islamabad, Pakistan. sohail.dagiwal@gmail.com

Applied Optics
|June 3, 2010
PubMed
Summary

Optical imaging resolution is enhanced using a novel mask-based approach. This method overcomes limitations from Charge-Coupled Device (CCD) pixel separation without moving parts, improving geometric superresolution.

More Related Videos

Super-resolution Imaging of the Bacterial Division Machinery
08:47

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

Related Experiment Videos

Last Updated: Jun 12, 2026

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform
06:25

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Super-resolution Imaging of the Bacterial Division Machinery
08:47

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy
14:23

Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy

Published on: March 6, 2018

Area of Science:

  • Optical Imaging
  • Computational Photography
  • Image Resolution Enhancement

Background:

  • Optical imaging resolution is constrained by lens aberrations and Charge-Coupled Device (CCD) pixel characteristics.
  • Specifically, the separation between CCD pixels significantly limits the achievable image resolution.
  • Existing methods often involve mechanical components, which can be complex and introduce other limitations.

Purpose of the Study:

  • To develop a novel method for achieving geometric superresolution in optical imaging.
  • To overcome the resolution limitations imposed by CCD pixel separation without using moving elements.
  • To introduce a mask-based encoding and decoding strategy for enhanced image capture.

Main Methods:

  • A mask is placed at the Fourier transform plane to encode input image data.
  • The encoded image is captured by a Charge-Coupled Device (CCD).
  • The captured image undergoes Fourier transformation, followed by the application of a decoding mask to counteract undersampling effects.

Main Results:

  • The proposed method demonstrates the potential for exceeding the geometric resolution limits imposed by CCD pixel separation.
  • Mathematical modeling and one-dimensional simulations validate the effectiveness of the mask-based approach.
  • The technique achieves superresolution without requiring any moving parts within the imaging system.

Conclusions:

  • A mask-based optical imaging technique can effectively enhance geometric resolution beyond CCD pixel limitations.
  • This approach offers a non-mechanical solution for superresolution, simplifying imaging system design.
  • The presented mathematical framework and simulations provide a foundation for further development and application.