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Three-dimensional Optical-resolution Photoacoustic Microscopy
Published on: May 3, 2011
Photoacoustic waves generated in blood studied using pulsed digital holography
Erik Olsson1, Per Gren, Mikael Sjödahl
1Division of Experimental Mechanics, Luleå University of Technology, SE-97187 Luleå, Sweden. erka@ltu.se
Applied Optics
|June 3, 2010
Summary
Pulsed digital holography visualized photoacoustic waves from reindeer blood. The study quantified acoustic properties, confirming pressure generation at the blood surface, consistent with existing data for oxygenated blood.
Area of Science:
- Biomedical Optics
- Acoustics
- Laser Physics
Background:
- Photoacoustic imaging relies on detecting acoustic waves generated by laser absorption.
- Pulsed digital holography offers a non-invasive method for wave visualization.
Purpose of the Study:
- To investigate photoacoustic wave generation and propagation in reindeer blood.
- To quantitatively analyze acoustic wave properties using pulsed digital holography.
- To validate the methodology by comparing results with known data for oxygenated blood.
Main Methods:
- Photoacoustic waves were generated in reindeer blood using a 1064 nm infrared laser pulse.
- Acoustic waves were imaged in water via pulsed digital holography with a 532 nm laser pulse.
- The inverse Radon transform was applied to recorded projections for quantitative analysis.
Main Results:
- The generated pressure pulse exhibited a flat, sharp front, indicating surface generation.
- Acoustic pressure was found to be proportional to laser fluence.
- The calculated proportionality constant (1.8+/-0.3 cm(-1)) closely matched established values for oxygenated blood (1.4 cm(-1)).
Conclusions:
- Pulsed digital holography is a viable technique for quantitative photoacoustic wave analysis.
- The study confirms surface-based photoacoustic pressure generation in blood.
- The findings support the accuracy of the employed holographic method for biological tissue studies.
