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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
07:34

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Rapid spectrally encoded fluorescence imaging using a wavelength-swept source.

Mathias Strupler1, Etienne De Montigny, Dominic Morneau

  • 1Engineering Physics Department, Ecole Polytechnique Montréal,2900, Boulevard Edouard-Montpetit, Montreal, Quebec, H3C3A7, Canada.

Optics Letters
|June 3, 2010
PubMed
Summary

We developed rapid spectral encoding (SE) for high-resolution fluorescence imaging. This technique achieves fast acquisition rates, enabling detailed visualization of samples like quantum dots, with potential for endoscopic applications.

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A Multimodal Wide-Field Fourier-Transform Raman Microscope
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A Multimodal Wide-Field Fourier-Transform Raman Microscope

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Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
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Area of Science:

  • Biomedical optics
  • Fluorescence imaging
  • Nanotechnology

Background:

  • Current fluorescence imaging methods face limitations in speed and resolution.
  • Spectral encoding (SE) offers potential for faster imaging but can be affected by sample properties like Stokes shifts.
  • Developing rapid, high-resolution imaging techniques is crucial for advanced diagnostics and research.

Purpose of the Study:

  • To present a rapid imaging technique using spectral encoding (SE) for fluorescent samples.
  • To demonstrate the preservation of SE position information despite Stokes shifts using a near-infrared wavelength-swept source.
  • To validate the technique's performance by imaging lead sulfide (PbS) quantum dot solutions.

Main Methods:

  • Utilized a near-infrared wavelength-swept source for spectral encoding (SE).
  • Implemented a simple configuration to preserve SE position information, overcoming Stokes shifts.
  • Imaged fluorescent lead sulfide (PbS) quantum dot solutions at various concentrations.

Main Results:

  • Achieved high-resolution imaging with acquisition rates up to 9920 lines of 1024 pixels per second.
  • Successfully imaged PbS quantum dot solutions at concentrations as low as 0.5±0.1 micromol/L.
  • Demonstrated the robustness of the SE technique in preserving positional information.

Conclusions:

  • The presented spectrally encoded setup enables rapid, high-resolution fluorescence imaging.
  • The technique is suitable for imaging low-concentration fluorescent samples, including quantum dots.
  • Miniaturization for endoscopy is feasible, combining fluorescence and confocal reflectance imaging at high speeds.