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Related Experiment Videos

Ranitidine interference with the monoclonal EMIT d.a.u. amphetamine/methamphetamine immunoassay.

A Poklis1, K V Hall, J Still

  • 1Medical College of Virginia Hospital, Department of Pathology, Virginia Commonwealth University, Richmond 23298-0597.

Journal of Analytical Toxicology
|March 1, 1991
PubMed
Summary

Ranitidine can cause false positive results in monoclonal EMIT d.a.u. amphetamine/methamphetamine immunoassays (ME). This interference, observed at ranitidine concentrations above 91 mg/L, did not affect polyclonal assays.

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Area of Science:

  • Clinical Chemistry
  • Forensic Toxicology
  • Pharmaceutical Analysis

Background:

  • Ranitidine is a widely prescribed medication.
  • Urine drug screening is crucial for patient management and legal purposes.
  • Immunoassays are common methods for drug detection but can be subject to interference.

Purpose of the Study:

  • To investigate the potential interference of ranitidine with a specific monoclonal immunoassay for amphetamine and methamphetamine.
  • To determine the concentration threshold at which ranitidine causes false positive results.

Main Methods:

  • Urine specimens from patients taking ranitidine were analyzed.
  • Ranitidine concentrations were measured using high-pressure liquid chromatography (HPLC).
  • Urine samples were tested using the monoclonal EMIT d.a.u. amphetamine/methamphetamine immunoassay (ME).

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Main Results:

  • Ranitidine was detected in patient urine at concentrations ranging from 7-271 mg/L.
  • False positive ME results were observed in samples with ranitidine concentrations exceeding 91 mg/L.
  • 12 out of 63 patient samples yielded false positive ME results, primarily in early voids post-ingestion.

Conclusions:

  • Ranitidine can interfere with the monoclonal EMIT d.a.u. amphetamine/methamphetamine immunoassay, leading to false positives.
  • The interference is concentration-dependent, occurring above 91 mg/L.
  • Polyclonal amphetamine assays (EMIT d.a.u. and TDx) were not affected by ranitidine interference.