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Related Experiment Videos

Intracellular calcium modulates gallbladder ion transport.

J A Cates1, K D Saunders, M Z Abedin

  • 1Research Service, Sepulveda VAMC, California.

The Journal of Surgical Research
|June 1, 1991
PubMed
Summary

Altered intracellular calcium ([Ca2+]ic) levels can affect gallbladder ion transport. Extracellular calcium

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Calmodulin regulation of gallbladder ion transport becomes dysfunctional during gallstone formation in prairie dogs.

Digestive diseases and sciences·2000

Area of Science:

  • Physiology
  • Cell Biology
  • Gastroenterology

Background:

  • Gallstone formation is linked to gallbladder absorption and biliary calcium.
  • Extracellular calcium ([Ca2+]ec) influences gallbladder ion transport.
  • The role of intracellular calcium ([Ca2+]ic) in this process is unclear.

Purpose of the Study:

  • To investigate the effects of altered intracellular calcium ([Ca2+]ic) on gallbladder (GB) ion transport.

Main Methods:

  • Prairie dog gallbladders were mounted in a Ussing chamber.
  • Short-circuit current (Isc), potential difference (Vms), and resistance (Rt) were measured.
  • Mucosal surfaces were treated with Dantrolene (Dt) or nickel (Ni2+) to manipulate intracellular calcium.

Main Results:

  • Dantrolene (Dt) caused transient reductions in Isc and Vms, suggesting a role for intracellular calcium.
  • Nickel (Ni2+) induced sustained decreases in Isc and Vms, indicating blocked extracellular calcium influx.
  • These findings support the hypothesis that intracellular calcium modulates GB ion transport.

Conclusions:

  • Intracellular calcium ([Ca2+]ic) may act as a modulator of gallbladder ion transport.
  • Observed effects of extracellular calcium ([Ca2+]ec) on ion transport might be mediated by intracellular calcium changes.

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