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Related Experiment Video

Updated: Jun 12, 2026

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

Microfluidic biolector-microfluidic bioprocess control in microtiter plates.

Matthias Funke¹, Andreas Buchenauer, Uwe Schnakenberg

¹AVT Biochemical Engineering, RWTH Aachen University, Worringerweg 1, 52074 Aachen, Germany.

Biotechnology and Bioengineering

|June 3, 2010

Summary

Researchers developed a novel microfermentation system for high-throughput, controlled cell cultivation. This system enables precise pH and substrate control in small volumes, overcoming scale-up challenges in biotechnology.

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Last Updated: Jun 12, 2026

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Published on: September 23, 2013

Area of Science:

Biotechnology
Bioprocess Engineering
Microfluidics

Background:

Industrial-scale cell cultivation relies on controlled pH and fed-batch strategies.
Small-scale cultivations typically use simple batch methods without process control.
This discrepancy hinders scale-up and scale-down due to altered microbial metabolism and kinetics.

Purpose of the Study:

To introduce a novel microfermentation system for high-throughput, controlled small-scale cultivations.
To address the limitations of current small-scale batch experiments.
To enable precise process control in microscale fermentation.

Main Methods:

Integration of a fiber-optic online-monitoring device (BioLector technology) with microfluidic chips.
Implementation of a micropump for controlled substrate feeding (fed-batch) in volumes below 1 mL.
Application in fermentations of Escherichia coli under pH-controlled and fed-batch conditions in shaken microtiter plates (MTPs).

Main Results:

A user-friendly microfermentation system providing high information output on a microscale.
Demonstration of parallelized and automated experiments using MTPs.
Successful application in controlled Escherichia coli fermentations.

Conclusions:

The novel microfermentation system offers a simple, high-throughput solution for controlled small-scale cultivations.
It bridges the gap between small-scale batch experiments and industrial-scale processes.
The system is easily transferable to disposable single-use applications.