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Related Concept Videos

Other Unique Bacteria01:18

Other Unique Bacteria

Magnetic bacteria exhibit a directed movement called magnetotaxis, driven by structures called magnetosomes. These magnetosomes consist of chains of magnetic particles made of either magnetite (Fe₃O₄) or greigite (Fe₃S₄) and are organized in a linear conformation by a protein scaffold within invaginations of the cell membrane. The bacteria align along the north–south magnetic field lines, much like a compass needle. They are typically microaerophilic or anaerobic and are commonly found near the...
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibody Structure01:10

Antibody Structure

Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibody Structure and Classes01:25

Antibody Structure and Classes

Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
Magnetism01:30

Magnetism

Magnets are commonly found in everyday objects, such as toys, hangers, elevators, doorbells, and computer devices. Experimentation on these magnets shows that all magnets have two poles: one is labeled north (N) and the other south (S). Magnetic poles repel if they are alike and attract if unlike. Moreover, both poles of a magnet attract unmagnetized pieces of iron.
An individual magnetic pole cannot be isolated. No matter how small, every piece of a magnet contains a north pole and a south...
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Updated: Jun 12, 2026

Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
06:48

Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads

Published on: September 15, 2016

Magnetizable antibody-like proteins.

Prabhjyot Kaur Dehal1, Christine Frances Livingston, Claire Geekie Dunn

  • 1Research and Development, Axis-Shield Diagnostics Ltd, The Technology Park, Dundee, UK. Prabhjyot.Dehal@axis-shield.com

Biotechnology Journal
|June 3, 2010
PubMed
Summary

Researchers developed novel magnetized fusion proteins using ferritin and antibody fragments for molecule isolation. These "organic" magnetic particles offer potential advantages over conventional inorganic magnetic beads.

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Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
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Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads

Published on: September 15, 2016

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles
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Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
06:58

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Area of Science:

  • Biotechnology
  • Materials Science
  • Immunology

Background:

  • Paramagnetic particles are widely used for isolating molecules and cells.
  • Traditional methods involve coating inorganic particles with biorecognition molecules.
  • Existing techniques have limitations that can be overcome with novel approaches.

Purpose of the Study:

  • To design and assess novel magnetized fusion proteins for enhanced molecule isolation.
  • To create "organic" magnetic particles with specific antigen-binding capabilities.
  • To explore the advantages of these new particles over traditional inorganic magnetic beads.

Main Methods:

  • Engineered fusion proteins combining antibody single chain variable fragments (scFv) with ferritin.
  • Expressed and assembled fusion proteins in E. coli.
  • Chemically magnetized the fusion proteins by incorporating a paramagnetic iron core.

Main Results:

  • Successfully produced magnetizable fusion proteins with surface-displayed scFvs.
  • Demonstrated the ability of the magnetized fusion proteins to bind target antigens.
  • The "organic" magnetic particles exhibited magnetizability and specific binding.

Conclusions:

  • Developed a novel class of "organic" magnetizable particles for biomolecule isolation.
  • These fusion protein-based magnetic particles show promise for various applications.
  • The designed particles offer theoretical advantages over conventional inorganic magnetic separation methods.