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Updated: Jun 12, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
[Effects of mangiferin on cell cycle status and CDC2/Cyclin B1 expression of HL-60 cells]
Yi-Bin Yao1, Zhi-Gang Peng, Zhen-Fang Liu
1Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China. yaoyiyi6@126.com
Objective:
To study the effects of mangiferin on cell cycle status and CDC2/Cyclin B1 expression in HL-60 cells, and its molecular mechanism for treating leukemia.
Methods:
The effect of Mangiferin on HL-60 cells proliferation was determined by MTT assay; The change of cell cycle status in HL-60 cells treated with mangiferin was performed by the flow cytometry; The expressions of CDC2 mRNA and Cyclin B1 mRNA were detected by semiquantitative RT-PCR.
Results:
The growth inhibition effects of mangiferin in HL-60 cells were enhanced as the mangiferin concentration increased and exposure time prolonged; HL-60 cells in G2/M phase increased in a dose-dependent way 24 hours after mangiferin administration, indicating G2/M phase blockage; the expressions of CDC2 mRNA and Cyclin B1 mRNA enhanced in a dose-dependent way and came to the peak at 80 micromol/L mangiferin.
Conclusion:
Mangiferin can inhibit the proliferation of HL-60, block the cell cycle progression in G2/M phase, and it can significantly increase the expressions of CDC2 mRNA and Cyclin B1 mRNA of HL-60 cells. G2/M phase blockage may be one of its molecular mechanism for treating leukemia.
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