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Updated: Jun 12, 2026
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
[Application of a simple method for detection of rubella virus genome by loop-mediated isothermal amplification
Shuang Wang1, Jian-hui Zhou, Xiang Hou
1Jilin Provincial Center for Disease Control and Prevention, Changchun 130062, Jilin, China.
Objective:
To use a new simple Reverse Transcription Loop-mediated isothermal amplification (RT-LAMP) method was applied to detect rubella virus nucleic acid and compared with Reverse Transcription-Polymerase Chain Reaction (RT-PCR).
Method:
Comparing the detection rate of the RT-LAMP method with that of RT-PCR for detecting rubella virus nucleic acid from rubella virus.
Results:
The nucleic acid positive rates of all 11 strains of rubella virus were 100% by the two methods, the positive rate was 55%.
Conclusion:
RT-LAMP is more simple and convenient than RT-PCR.
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