Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Low-molecular-weight polysaccharide and polyol impregnation enhances the rehydration recovery capacity of freeze-dried potato slices.

Food chemistry: X·2026
Same author

Lignin hydrogels: From materials and properties to integrated monitoring-diagnosis-therapy applications: A review.

International journal of biological macromolecules·2026
Same author

The NAC Transcription Factor SlNAP2 Enhances Tomato Resistance to Ralstonia solanacearum.

Physiologia plantarum·2026
Same author

Dynamics of Singlet Fission in the TIPS-Pn Cluster: Endothermic or Exothermic?

The journal of physical chemistry letters·2026
Same author

Hydroxypropylated/oxidized starch-based regulation of the potato-barley system for extrusion-based 3D food printing.

International journal of biological macromolecules·2026
Same author

Patient preferences and willingness-to-pay for therapy in generalized myasthenia gravis: a large-scale discrete choice experiment in China.

Frontiers in immunology·2026

Related Experiment Video

Updated: Jun 12, 2026

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

[Construction and identification of different stem shRNA expression vectors].

Zhonghua Liu1, Xianfeng Qiao, Hongwei Xiao

  • 1Shaanxi Stem Cell Engineering and Technology Research Center, Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Veterinary Medicine, Northwest A & F University, Yangling 712100, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|June 4, 2010
PubMed
Summary

This study tested short hairpin RNA (shRNA) vectors with varying stem lengths for gene silencing in mouse cells. Longer shRNA stems, specifically 29 base pairs, demonstrated superior gene silencing effectiveness compared to shorter ones.

More Related Videos

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
12:59

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction
04:18

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction

Published on: May 17, 2024

Related Experiment Videos

Last Updated: Jun 12, 2026

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
12:59

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction
04:18

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction

Published on: May 17, 2024

Area of Science:

  • Molecular Biology
  • RNA Interference

Context:

  • Short hairpin RNA (shRNA) technology is crucial for gene silencing.
  • Optimizing shRNA design is essential for effective gene knockdown.
  • Understanding the impact of stem length on shRNA efficacy is key.

Purpose:

  • To investigate the relationship between shRNA stem length and gene silencing efficiency in mouse models.
  • To compare the effectiveness of shRNA vectors with 21 bp, 27 bp, and 29 bp stems targeting the enhanced green fluorescent protein (EGFP) gene.

Summary:

  • shRNA vectors with varying stem lengths (21 bp, 27 bp, 29 bp) were constructed and tested for silencing the EGFP gene in mouse embryonic fibroblasts.
  • Gene silencing was assessed using real-time quantitative PCR to measure EGFP mRNA levels.
  • The 29 bp shRNA vector exhibited significantly stronger gene silencing activity than the 21 bp and 27 bp vectors.

Impact:

  • Provides valuable insights for selecting optimal shRNA hairpin designs for gene silencing applications in mice.
  • Contributes to the understanding of structure-activity relationships in RNA interference.
  • Informs the development of more efficient gene silencing tools for research and potential therapeutic applications.