Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Production of Organic Acids01:25

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
Hydrolysis01:15

Hydrolysis

Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thermostable Bioluminescent Intercalating Dyes for Real-Time, Integrated Nucleic Acid Amplification and Detection.

Angewandte Chemie (International ed. in English)·2026
Same author

Redesign of TALE proteins for DNA-templated assembly of protein fibers.

Nature communications·2026
Same author

Silk-Inspired Design and Manufacturing of Robust Plantymers.

Nature communications·2026
Same author

Activity of ice-binding proteins can be markedly enhanced by protein tags.

Nanoscale·2026
Same author

Functionalised plant protein powders: From phase separation to particulate isolates.

Food research international (Ottawa, Ont.)·2025
Same author

Inhibition of ice recrystallization with designed twistless helical repeat proteins.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Jun 12, 2026

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
08:43

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides

Published on: June 20, 2010

Lysozyme uptake by oxidized starch polymer microgels.

Yuan Li1, Renko de Vries, Mieke Kleijn

  • 1Laboratory of Physical Chemistry and Colloid Science, Dreijenplein 6, 6703 HB Wageningen, The Netherlands. yuan3.li@wur.nl

Biomacromolecules
|June 4, 2010
PubMed
Summary

This study reveals optimal conditions for protein binding to oxidized potato starch polymer (OPSP) microgels. The best pH for protein uptake and release is around 5, balancing high binding capacity and affinity.

More Related Videos

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
11:31

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

Published on: September 15, 2015

Molecular Entanglement and Electrospinnability of Biopolymers
07:59

Molecular Entanglement and Electrospinnability of Biopolymers

Published on: September 3, 2014

Related Experiment Videos

Last Updated: Jun 12, 2026

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
08:43

OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides

Published on: June 20, 2010

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
11:31

High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release

Published on: September 15, 2015

Molecular Entanglement and Electrospinnability of Biopolymers
07:59

Molecular Entanglement and Electrospinnability of Biopolymers

Published on: September 3, 2014

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Protein Chemistry

Background:

  • Microgels are widely used in drug delivery and biomaterials.
  • Understanding protein-microgel interactions is crucial for optimizing their applications.
  • Oxidized potato starch polymer (OPSP) microgels offer unique properties for protein binding.

Purpose of the Study:

  • To determine optimal conditions for globular protein uptake and release using OPSP microgels.
  • To investigate the influence of pH and ionic strength on protein-microgel interactions.
  • To characterize the binding capacity and affinity of lysozyme to OPSP microgels.

Main Methods:

  • Studied the interaction between OPSP microgel particles and hen egg lysozyme.
  • Analyzed protein-induced deswelling behavior of charged microgels.
  • Investigated the effect of varying pH and salt concentrations on protein uptake and release.

Main Results:

  • Protein distribution within the microgel was found to be homogeneous.
  • Protein uptake increased with pH at low salt concentrations due to charge compensation.
  • Protein binding affinity decreased sharply with increasing pH.
  • Protein release was facilitated at high pH and by adding salt.
  • Maximum protein uptake (Gammasat) shifted to lower pH with increasing ionic strength.

Conclusions:

  • The OPSP microgel system demonstrates tunable protein binding properties.
  • Optimal conditions for protein uptake and release are achieved around pH 5.
  • This system shows promise for applications requiring controlled protein adsorption and desorption.