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Published on: March 30, 2019
Inhibitory effect of HuR gene small interfering RNA segment on laryngeal carcinoma Hep-2 cell growth
1Department of Otorhinolaryngology-Head and Neck Surgery, Lihuili Hospital of Ningbo University, China. szs7216@sina.com
Objectives:
To investigate the effect of the HuR gene on laryngeal carcinoma Hep-2 cell growth, and to analyse correlations between the HuR, cyclooxygenase-2 and survivin genes.
Study Design:
Experiment study.
Setting:
Department of Otolaryngology-Head and Neck Surgery, Lihuili Hospital of Ningbo University, Ningbo, a tertiary care centre in China.
Methods:
Copies of a small interfering RNA segment directed against the HuR gene were transfected into Hep-2 cells using Lipofectamine™ 2000. The effect of the small interfering RNA segment on Hep-2 cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Changes in the expression of the HuR, cyclooxygenase-2 and survivin genes were detected by semi-quantitative reverse transcription polymerase chain reaction analysis. Concentrations of the HuR, cyclooxygenase-2 and survivin proteins were evaluated using Western blotting.
Results:
Expression of the HuR, cyclooxygenase-2 and survivin genes, as indicated by messenger RNA and protein levels, was suppressed by the HuR gene small interfering RNA segment in a dose-dependent manner. The proliferation indices of all treated groups were significantly lower than those of control groups (p < 0.05).
Conclusions:
Impairment of HuR gene expression, using interfering RNA technology, can significantly suppress Hep-2 cell proliferation and induce apoptosis. The HuR gene may be an effective target for gene therapy in patients with laryngeal carcinoma.
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