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Updated: Jun 12, 2026

Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization
Published on: October 3, 2025
R spider: a network-based analysis of gene lists by combining signaling and metabolic pathways from Reactome and KEGG
Alexey V Antonov1, Esther E Schmidt, Sabine Dietmann
1Helmholtz Zentrum München-German Research Center for Environmental Health (GmbH), Institute for Bioinformatics and Systems Biology, Ingolstädter Landstrasse 1, D-85764 Neuherberg, Germany. alexey.antonov@helmholtz-muenchen.de
Abstract:
R spider is a web-based tool for the analysis of a gene list using the systematic knowledge of core pathways and reactions in human biology accumulated in the Reactome and KEGG databases. R spider implements a network-based statistical framework, which provides a global understanding of gene relations in the supplied gene list, and fully exploits the Reactome and KEGG knowledge bases. R spider provides a user-friendly dialog-driven web interface for several model organisms and supports most available gene identifiers. R spider is freely available at http://mips.helmholtz-muenchen.de/proj/rspider.
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