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Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

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Related Experiment Video

Updated: Jun 12, 2026

Pyrosequencing for Microbial Identification and Characterization
12:37

Pyrosequencing for Microbial Identification and Characterization

Published on: August 22, 2013

Analyzing endodontic infections by deep coverage pyrosequencing.

L Li1, W W L Hsiao, R Nandakumar

  • 1Department of Endodontics, Prosthodontics and Operative Dentistry, Dental School, University of Maryland, 650 W. Baltimore St., Baltimore, MD 21201, USA.

Journal of Dental Research
|June 4, 2010
PubMed
Summary
This summary is machine-generated.

Pyrosequencing reveals greater bacterial diversity in endodontic infections than previously known. This advanced method, using 16S rRNA gene sequencing, identified more bacterial genera and low-abundance microbes with potential clinical significance.

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Published on: May 2, 2018

Area of Science:

  • Microbiology
  • Genomics
  • Dental Research

Background:

  • Bacterial diversity in endodontic infections remains underexplored.
  • Traditional Sanger sequencing offers limited depth for comprehensive analysis.

Purpose of the Study:

  • To investigate and compare bacterial diversity in endodontic infections using pyrosequencing and Sanger sequencing.
  • To identify bacterial genera and phyla, including low-abundance organisms, present in endodontic infections.

Main Methods:

  • Utilized 454 GS-FLX pyrosequencing and conventional Sanger sequencing on 7 endodontic infection specimens.
  • Performed taxonomic classification of bacterial communities based on 16S rRNA gene sequences.
  • Analyzed sequence data using Ribosomal Database Project (RDP II) Classifier.

Main Results:

  • Pyrosequencing generated significantly more sequences per sample (28,590) compared to Sanger sequencing (47), a 600-fold difference in depth-of-coverage.
  • Pyrosequencing identified 179 bacterial genera across 13 phyla, substantially exceeding Sanger sequencing's findings.
  • The phylum Bacteroidetes was identified as the most prevalent bacterial phylum in the studied samples.

Conclusions:

  • Bacterial communities in endodontic infections are considerably more diverse than previously understood.
  • Deep-coverage pyrosequencing of the 16S rRNA gene is crucial for detecting low-abundance microorganisms.
  • The findings highlight potential clinical implications of previously undetected microbial diversity in endodontic infections.