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[Reverse plaque formation by hog cholera virus inducing interference with VSV (author's transl)].
Archives of Virology
|January 1, 1978
Summary
Hog cholera virus (HCV) infection makes PK15 cells temporarily resistant to VSV. The reverse plaque assay effectively quantifies different HCV strains, aiding in diagnostics and research.
Area of Science:
- Virology
- Cell Biology
- Infectious Diseases
Background:
- Hog cholera virus (HCV), a togavirus, causes significant disease in swine.
- Understanding viral interference mechanisms is crucial for controlling infectious diseases.
- PK15 cells are a commonly used cell line for swine virus research.
Purpose of the Study:
- To investigate the effect of HCV infection on PK15 cell susceptibility to vesicular stomatitis virus (VSV).
- To evaluate the utility of the reverse plaque procedure for titrating various HCV strains.
Main Methods:
- PK15 cells were infected with different HCV strains.
- VSV challenge assays were performed to assess cellular refractivity.
- The reverse plaque assay was employed for HCV titration.
Main Results:
- HCV infection induced a transient refractory state in PK15 cells, limiting VSV replication.
- The reverse plaque procedure proved effective for titrating virulent, chronic, and attenuated HCV strains.
Conclusions:
- HCV infection confers temporary resistance to VSV in PK15 cells.
- The reverse plaque assay is a practical method for quantifying diverse HCV strains.