Related Experiment Video
Updated: Jun 12, 2026

Visualizing Bacteria in Nematodes using Fluorescent Microscopy
Published on: October 19, 2012
A series of IncQ-based reporter plasmids for use in a range of Gram negative genera
Laura E O'Sullivan1, Cheryl A Nickerson, James W Wilson
1Department of Biology, Villanova University, Villanova, PA 19085, USA.
Abstract:
Many studies require expression analysis of the same gene/promoter across a range of bacterial genera. However, there is currently a lack of availability of reporters based on the broad host range IncQ replicon, which is compatible with a popular improved IncP transfer system that is self-transfer defective. We report IncQ lacZ reporter plasmids with features including: (1) compatibility with IncP, IncW, and pBHR/pBBR replicons, (2) a variety of antibiotic markers (Sp-r, Sm-r, Km-r, Cm-r), (3) convenient mobilization via a novel self-transfer-defective IncP conjugation system, and (4) complete DNA sequences. Utility is demonstrated using three different promoters in different Gram negative genera.
Related Concept Videos
Plasmids
Antibiotic Selection
Gene Regulation in Microbial Communities: Quorum Sensing

