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Related Concept Videos

Mutations in Microorganisms01:18

Mutations in Microorganisms

Mutations are heritable changes in an organism’s genome involving alterations in the base sequence of DNA or RNA. These changes can influence cellular processes and phenotypic traits, potentially transforming the unaltered wild type into a mutant form. Such changes, termed forward mutations, are pivotal in shaping the genetic diversity of organisms.RNA viruses exhibit the highest mutation rates due to the absence of robust proofreading mechanisms during genome replication. In contrast,...
Export of Mitochondrial and Chloroplast Genes02:19

Export of Mitochondrial and Chloroplast Genes

A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred irrespective...

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Related Experiment Video

Updated: Jun 12, 2026

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
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Published on: March 7, 2019

S. pombe genome deletion project: an update.

Mario Spirek1, Zsigmond Benko, Martina Carnecka

  • 1Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.

Cell Cycle (Georgetown, Tex.)
|June 4, 2010
PubMed
Summary

Researchers created a comprehensive collection of fission yeast gene deletion mutants. This nearly complete set of Schizosaccharomyces pombe deletion strains advances eukaryotic biology studies.

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Area of Science:

  • * Molecular and Cellular Biology
  • * Genetics and Genomics
  • * Yeast Biology

Background:

  • * Schizosaccharomyces pombe (fission yeast) is a key model organism for eukaryotic cell biology.
  • * Previous efforts created a large deletion mutant collection using PCR-based methods.
  • * Some genes remained undeleted due to limitations in existing methodologies.

Purpose of the Study:

  • * To expand the existing collection of fission yeast deletion mutants.
  • * To achieve near-complete coverage of the Schizosaccharomyces pombe genome.
  • * To develop an efficient gene knockout strategy for difficult-to-delete genes.

Main Methods:

  • * Employed a plasmid-based knockout strategy.
  • * Utilized plasmids containing large homologous regions for efficient gene targeting.
  • * Targeted an additional 29 genes for deletion in Schizosaccharomyces pombe.

Main Results:

  • * Successfully deleted 29 additional genes in fission yeast.
  • * Developed an efficient and effective gene knockout method.
  • * Expanded the deletion mutant collection to cover 99% of open reading frames.

Conclusions:

  • * The enhanced gene deletion strategy significantly improved mutant collection coverage.
  • * The near-complete collection of Schizosaccharomyces pombe deletion mutants is a valuable resource.
  • * This resource will accelerate research in eukaryotic cell biology and genetics.