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MRI and PET in Mouse Models of Myocardial Infarction
Published on: December 19, 2013
Image-derived input function from cardiac gated maximum a posteriori reconstructed PET images in mice
Landon W Locke1, Stuart S Berr, Bijoy K Kundu
1Department of Biomedical Engineering, The University of Virginia, P.O. Box 801339, 480 Ray C Hunt Drive, Snyder Building, Charlottesville, VA 22908, USA.
Molecular Imaging and Biology
|June 4, 2010
Summary
Accurate image-derived input functions (IDIF) can be measured non-invasively from cardiac gated positron emission tomography (PET) images. This method, using OSEM-MAP reconstruction, eliminates the need for further corrections, simplifying analysis.
Area of Science:
- Nuclear medicine
- Medical imaging
- Pharmacokinetics
Background:
- Accurate input functions are crucial for quantitative analysis in positron emission tomography (PET).
- Traditional methods rely on invasive blood sampling, which can be cumbersome and introduce variability.
- Image-derived input functions (IDIF) offer a non-invasive alternative but require validation.
Purpose of the Study:
- To determine if accurate IDIFs can be obtained from cardiac gated PET images reconstructed with OSEM-MAP without additional corrections.
- To assess the accuracy of IDIFs against traditional blood sampling methods.
- To evaluate the impact of non-invasive IDIFs on kinetic modeling in myocardial glucose uptake studies.
Main Methods:
- Cardiac gated PET images were acquired from C57/BL6 mice.
- Images were reconstructed using ordered subset expectation maximization-maximum a posteriori (OSEM-MAP) with CT-based attenuation correction.
- IDIFs were extracted from the left ventricle and compared to blood samples using Bland-Altman analysis and paired t-tests.
Main Results:
- Image-derived radioactivity concentrations showed no significant difference compared to sampled blood values at late time points (P=0.97).
- Bland-Altman analysis indicated a minimal mean difference of 1% between IDIF and blood samples.
- Kinetic analysis using IDIFs yielded myocardial 2-deoxy-2-[18F]fluoro-D-glucose uptake rate constants comparable to literature values.
Conclusions:
- Accurate, non-invasive IDIFs can be successfully derived from cardiac gated PET images reconstructed with OSEM-MAP and attenuation correction.
- No further spillover corrections are required for these IDIFs, simplifying the analysis pipeline.
- This approach enhances the feasibility of quantitative cardiac PET studies without compromising accuracy.
