Related Experiment Video
Updated: Jun 12, 2026
Murine Model of Allergen Induced Asthma
Published on: May 14, 2012
Eosinophils are required for the induction of bronchial hyperresponsiveness in a Th transfer model of BALB/c
Takayuki Ohtomo1, Osamu Kaminuma, Junji Yamada
1National Hospital Organization, Sagamihara National Hospital, Clinical Research Center for Allergy and Rheumatology, Kanagawa, Japan.
Background:
Helper T (Th) cells are deeply involved in the pathophysiology of bronchial asthma, such as eosinophilic inflammation, bronchial hyperresponsiveness (BHR), airflow limitation and remodeling. It is still unclear whether Th cells contribute to BHR independently of eosinophilic inflammation. The double GATA (dblGATA) site is a high-affinity GATA-binding site in the GATA-1 promoter. dblGATA site-deficient (Delta dblGATA) mice lack eosinophils.
Method:
Ovalbumin (OVA)-reactive Th clones were transferred into Delta dblGATA and wild-type (WT) mice of BALB/c background. The number of eosinophils in the bronchoalveolar lavage fluid (BALF) and bronchial responsiveness to methacholine were examined after OVA challenge.
Results:
The number of BALF eosinophils was significantly increased in WT mice, but not detectable in Delta dblGATA mice. BHR was also induced in WT mice, but significantly attenuated in Delta dblGATA mice.
Conclusion:
Eosinophils are involved in T-cell-mediated BHR.
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