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Related Concept Videos

Membrane Fluidity01:26

Membrane Fluidity

Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is a relatively...
Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...

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Related Experiment Video

Updated: Jun 12, 2026

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy
08:39

Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

Published on: December 12, 2025

Watching single lipids move.

Natalie de Souza

    Nature Methods
    |June 5, 2010
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a high-resolution method using multiple photodiode detectors to track single dye-labeled lipids. This technique precisely monitors lipid transit through an excitation spot for advanced molecular studies.

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    Last Updated: Jun 12, 2026

    Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy
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    Tracking Single Proteins in Lipid Bilayers Using Fluorescence Microscopy

    Published on: December 12, 2025

    Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
    10:43

    Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes

    Published on: July 19, 2022

    Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy
    08:55

    Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy

    Published on: February 17, 2023

    Area of Science:

    • Biophysics
    • Analytical Chemistry
    • Molecular Imaging

    Background:

    • Single-molecule detection techniques are crucial for understanding cellular processes.
    • Current methods for tracking lipid dynamics often face limitations in resolution and speed.

    Discussion:

    • This research presents a novel application of multiple photodiode detectors for high-resolution tracking of single lipid molecules.
    • The method allows for precise monitoring of dye-labeled lipid transit through a defined excitation area.

    Key Insights:

    • Achieved high-resolution tracking of individual lipid molecules.
    • Demonstrated the capability to precisely measure lipid transit times.
    • Validated the use of photodiode arrays for sensitive molecular detection.

    Outlook:

    • Potential applications in studying lipid-protein interactions and membrane dynamics.
    • Further development could enhance throughput for large-scale lipidomics studies.
    • This technique may be adaptable for tracking other types of single molecules in biological systems.