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Pseudomonas aeruginosa in chronic suppurative otitis media: sensitivity spectrum against various antibiotics in
Tahira Mansoor1, Mohammed Ayub Musani, Gulnaz Khalid
1Department of Biotechnology, University of Karachi, Karachi, Pakistan.
Background:
Chronic suppurative otitis media (CSOM) is a prevailing and notorious infection in developing countries causing serious local damage and threatening complications. Early and effective treatment based on the knowledge of causing micro organisms and their sensitivity results in good clinical recovery and prevents from damage and complications. Pseudomonas aeruginosa is most common pathogen causing CSOM in Pakistan. The objective of this study is to identify incidence of Pseudomonas aeruginosa involved in CSOM and sensitivity against commonly prescribed antibiotics.
Methods:
A total of 263 patients with unilateral or bilateral chronic suppurative otitis media (CSOM) were enrolled in the study at Department of ENT, Karachi Medical and Dental College & Abbasi Shaheed Hospital, Karachi, Pakistan from December 2004 to May 2006. Sterile cotton swabs were used to collect pus from discharging ears and plated on Blood agar and MacConkey agar for 24 to 48 hours. Antibiotics susceptibility was tested by Minimum inhibitory concentrations (MIC) method using Mueller-Hinton agar.
Results:
Overall microbiology of 267 samples from 263 patients was studied including 4 of bilateral discharge. Polymicrobial growth was present in 8 samples. A total of 275 bacterial isolates were studied. Pseudomonas aeruginosa (40%) and Staphylococcus aureus (30.9%) were the most common bacterial agents found in CSOM. MIC was done for Pseudomonas aeruginosa only as it was the commonest pathogen found in CSOM. Sensitivity pattern of Pseudomonas aeruginosa showed that amikacin was active against 96% of isolates followed by ceftazidime 89%, ciprofloxacin 85%, gentamicin 81%, imipenem 76%, aztreonam 42% and ceftriaxone 21%.
Conclusions:
Pseudomonas aeruginosa was the most common bacteria isolated from chronic discharging ears followed by Staphylococcus aureus. Amikacin was found to be the most suitable drug followed by ceftazidime and ciprofloxacin for Pseudomonas aeruginosa. The resistance against ceftriaxone and aztreonam was found to be very high.
Insights
Pseudomonas aeruginosa is the most common cause of chronic suppurative otitis media (CSOM) in Pakistan. Amikacin, ceftazidime, and ciprofloxacin show the best sensitivity against this pathogen.
Area of Science:
- Otolaryngology
- Infectious Diseases
- Microbiology
Background:
- Chronic suppurative otitis media (CSOM) is a significant health issue in developing nations, leading to severe local damage and complications.
- Effective treatment of CSOM relies on identifying causative microorganisms and their antibiotic sensitivities.
- Pseudomonas aeruginosa is a prevalent pathogen in CSOM cases within Pakistan.
Purpose of the Study:
- To determine the incidence of Pseudomonas aeruginosa in CSOM cases.
- To evaluate the antibiotic susceptibility patterns of Pseudomonas aeruginosa isolates from CSOM patients.
Main Methods:
- A study of 263 patients with CSOM was conducted in Karachi, Pakistan.
- Pus samples were collected and cultured on Blood agar and MacConkey agar.
- Antibiotic susceptibility testing was performed using the Minimum Inhibitory Concentration (MIC) method.
Main Results:
- Pseudomonas aeruginosa was identified as the most common bacterial isolate (40%) in CSOM, followed by Staphylococcus aureus (30.9%).
- Amikacin demonstrated high activity against Pseudomonas aeruginosa (96% sensitivity), followed by ceftazidime (89%) and ciprofloxacin (85%).
- High resistance rates were observed for ceftriaxone (21%) and aztreonam (42%).
Conclusions:
- Pseudomonas aeruginosa is the primary bacterial agent in chronic discharging ears in the studied population.
- Amikacin, ceftazidime, and ciprofloxacin are recommended as effective treatment options for Pseudomonas aeruginosa infections in CSOM.
- Significant resistance to ceftriaxone and aztreonam necessitates careful consideration in treatment selection.
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