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Related Experiment Video

Updated: Jun 12, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Methods in DNA methylation profiling.

Tao Zuo1, Benjamin Tycko, Ta-Ming Liu

  • 1Division of Human Cancer Genetics, Department of Molecular Virology, Immunology and Medical Genetics, Comprehensive Cancer Center, Ohio State University, Columbus, OH 43210, USA. tim.huang@osumc.edu

Epigenomics
|June 8, 2010
PubMed
Summary
This summary is machine-generated.

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Analyzing genome-wide DNA methylation patterns is crucial for understanding genomic regulation. Combining multiple analytical methods provides the clearest view of the methylome, essential for future discoveries.

Area of Science:

  • Genomics
  • Epigenetics
  • Molecular Biology

Background:

  • DNA methylation is a key epigenetic mechanism regulating genome function.
  • Heritable and metastable DNA methylation patterns play significant roles in genomic regulation.
  • Understanding these patterns is vital for biological and disease research.

Purpose of the Study:

  • To review current methods for analyzing genome-wide DNA methylation patterns.
  • To provide a perspective on the existing literature for new investigators.
  • To stimulate future progress in epigenomic analysis.

Main Methods:

  • Review of historical and current analytical techniques for DNA methylation.
  • Discussion of various genome-wide profiling modalities.
Keywords:
CpG islandDNA methylationbisulfite sequencingdifferential methylation hybridizationmethylated CpG island recovery assaymethylated DNA immunoprecipitationmethylation profilingmethylation-sensitive SNP chip analysisnext-generation DNA sequencing

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Targeted DNA Methylation Analysis by Next-generation Sequencing
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Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Related Experiment Videos

Last Updated: Jun 12, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
13:11

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

  • Emphasis on the importance of integrating multiple analytical approaches.

    • Main Results:

    • No single method offers a complete view of the methylome.
    • Combining different analytical modalities provides the most comprehensive data.
    • Recent studies have uncovered novel epigenomic patterns and disease markers.

    Conclusions:

    • Genome-wide DNA methylation profiling is a rapidly advancing field.
    • Integrated analytical strategies are essential for maximizing insights.
    • Continued discoveries in epigenomics are expected, impacting biology and medicine.