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Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
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Strategies for kinome profiling in cancer and potential clinical applications: chemical proteomics and array-based

Sander R Piersma1, Mariette Labots, Henk M W Verheul

  • 1Department of Medical Oncology, OncoProteomics Laboratory, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands.

Analytical and Bioanalytical Chemistry
|June 8, 2010
PubMed

Insights

Kinase activity profiling in tumors can reveal aberrant signaling pathways crucial for cancer progression. This approach holds promise for personalized medicine, enabling patient stratification and targeted therapy selection.

Area of Science:

  • Biochemistry
  • Oncology
  • Molecular Biology

Background:

  • Kinases are crucial enzymes in signal transduction pathways.
  • Deregulated kinase activity is implicated in cancer development and progression.
  • Personalized medicine necessitates advanced diagnostic tools for patient stratification and therapy selection.

Purpose of the Study:

  • To explore kinome profiling for identifying tumor-specific aberrant signaling pathways.
  • To investigate the potential of kinome activity profiles for predicting therapy response.
  • To provide an outlook on implementing kinome profiling in clinical practice for therapy selection.

Main Methods:

  • Utilizing peptide array-based technologies for kinome profiling.
  • Employing mass spectrometry-based techniques for kinome analysis.
  • Describing new developments in kinome profiling technologies.

Main Results:

  • Kinome profiling can yield tumor-specific information on aberrant cell signaling.
  • Tumor tissue kinase activity profiles may correlate with therapy response.
  • The described technologies offer new avenues for kinome analysis.

Conclusions:

  • Kinome profiling is a valuable tool for understanding cancer signaling.
  • This approach has the potential to guide personalized therapy selection.
  • Future clinical implementation of kinome profiling is anticipated.

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