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Induction and Analysis of Epithelial to Mesenchymal Transition

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

SERPINB3 induces epithelial-mesenchymal transition.

Santina Quarta¹, Laura Vidalino, Cristian Turato

¹Department of Clinical and Experimental Medicine, University of Padova, Italy.

The Journal of Pathology

|June 8, 2010

Summary

The study shows that SERPINB3 protein promotes cancer cell invasion by triggering epithelial-mesenchymal transition (EMT). This process enhances tumor cell migration and metastasis, highlighting SERPINB3 as a potential therapeutic target.

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Induction and Analysis of Epithelial to Mesenchymal Transition

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Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

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Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

Area of Science:

Oncology
Cell Biology
Biochemistry

Background:

Epithelial-mesenchymal transition (EMT) is crucial for tumor invasion and metastasis.
SERPINB3, a serine protease inhibitor, is over-expressed in epithelial tumors and inhibits apoptosis.

Purpose of the Study:

To investigate the role of SERPINB3 in modulating EMT and its impact on cancer cell invasion.
To determine the autocrine and paracrine effects of SERPINB3 on tumor cell behavior.

Main Methods:

Morphological, molecular, and cell biology techniques were used on HepG2 cell clones transfected with SERPINB3.
Paracrine effects were assessed using exogenous recombinant SERPINB3 on HepG2 and MDCK cell lines.
Ultrastructural analysis and gene expression studies were performed.

Main Results:

SERPINB3 expression altered cell morphology, reduced desmosomal junctions, and increased intercellular spaces.
E-cadherin decreased while beta-catenin and vimentin increased, indicating EMT.
SERPINB3 induced cell scattering, migration, invasiveness, and colony formation in soft agar, independent of protease activity.

Conclusions:

SERPINB3 promotes EMT, leading to increased cell invasiveness and metastasis potential.
SERPINB3 acts through both autocrine and paracrine mechanisms.
These findings suggest SERPINB3 is a key player in tumor progression and a potential therapeutic target.