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Proteome changes in human monocytes upon interaction with calcium oxalate monohydrate crystals
Nilubon Singhto1, Kitisak Sintiprungrat, Supachok Sinchaikul
1Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Journal of Proteome Research
|June 10, 2010
Summary
Calcium oxalate crystals alter monocyte proteomes, affecting cell cycle and metabolism. This interaction may drive kidney stone disease inflammation via oxidative stress pathways.
Area of Science:
- Nephrology
- Immunology
- Proteomics
Background:
- Monocytes infiltrate kidneys around calcium oxalate (CaOx) deposits.
- Monocytes are thought to clear these crystals, but their response to CaOx is unknown.
Purpose of the Study:
- Investigate the proteomic changes in human monocytes upon interaction with calcium oxalate monohydrate (COM) crystals.
Main Methods:
- U937 human monocyte cells were cultured with or without COM crystals.
- Proteomic analysis was performed using 2D-gel electrophoresis (2-DE) and mass spectrometry (Q-TOF MS/MS).
- Differential protein expression was confirmed by Western blot.
Main Results:
- 22 differentially expressed proteins were identified (9 up-regulated, 13 down-regulated).
- Affected proteins are involved in cell cycle, cellular structure, metabolism, and protein processing.
- Confirmed changes include up-regulation of Alix and EF-2, and down-regulation of beta-actin.
Conclusions:
- COM crystal interaction significantly alters the monocyte proteome.
- These alterations may contribute to kidney stone disease pathogenesis through inflammatory pathways and oxidative stress.
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