Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Detectors for fluorescence microscopy.

K R Spring1

  • 1National Heart, Lung, and Blood Institute, Bethesda, MD 20892.

Scanning Microscopy
|March 1, 1991
PubMed
Summary
This summary is machine-generated.

Intensified video cameras improve low-light fluorescence microscopy by enabling better detection and measurement. Cooled CCD cameras are recommended for quantitative imaging in low-light conditions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Beta1-integrins in the primary cilium of MDCK cells potentiate fibronectin-induced Ca2+ signaling.

American journal of physiology. Renal physiology·2004
Same author

Bending the primary cilium opens Ca2+-sensitive intermediate-conductance K+ channels in MDCK cells.

The Journal of membrane biology·2003
Same author

Removal of the MDCK cell primary cilium abolishes flow sensing.

The Journal of membrane biology·2003
Same author

Solute recirculation.

The Journal of physiology·2002
Same author

Specific lectin binding to beta1 integrin and fibronectin on the apical membrane of madin-darby canine kidney cells.

The Journal of membrane biology·2002
Same author

Bending the MDCK cell primary cilium increases intracellular calcium.

The Journal of membrane biology·2001
Same journal

Deposition of supercoiled DNA on mica for scanning force microscopy imaging.

Scanning microscopy·1996
Same journal

Relative intranuclear magnesium and phosphorus contents in normal and tumor cells of the human thyroid gland as revealed by energy-dispersive X-ray microanalysis.

Scanning microscopy·1996
Same journal

Preparation of cultured smooth muscle cells from human myometrium for X-ray microanalysis.

Scanning microscopy·1996
Same journal

Combined bronchoalveolar-vascular casting of the canine lung.

Scanning microscopy·1996
Same journal

Effects of castration upon the morphology of the accessory sex organs of the male rat--a scanning electron microscopy study.

Scanning microscopy·1996
Same journal

Scanning electron microscopy of the accessory sex glands of the adult male rat.

Scanning microscopy·1996
See all related articles

Area of Science:

  • Microscopy
  • Biophysics
  • Cell Biology

Background:

  • Low light levels in fluorescence microscopy limit spatial and temporal resolution.
  • Detecting and measuring faint signals from living cells is challenging.

Purpose of the Study:

  • To compare the performance of intensified video cameras for low-light imaging.
  • To provide guidelines for selecting appropriate detectors for fluorescence microscopy.

Main Methods:

  • Evaluation of performance characteristics of popular intensified video cameras.
  • Comparison of camera suitability for various experimental requirements.

Main Results:

  • Intensified video cameras facilitate detection, visualization, and measurement of low-level fluorescence signals.

Related Experiment Videos

  • Performance characteristics of different cameras were assessed.

    • Conclusions:

    • Intensified or cooled CCD cameras are optimal for quantitative low-light imaging in fluorescence microscopy.
    • Selection guidelines aid researchers in choosing suitable detectors for their specific needs.