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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Model-based detection of alternative splicing signals.

Yoseph Barash1, Benjamin J Blencowe, Brendan J Frey

  • 1Banting and Best Department of Medical Research, University of Toronto, ON, Canada. yoseph@psi.utoronto.ca

Bioinformatics (Oxford, England)
|June 10, 2010
PubMed
Summary

This study introduces a new probabilistic model for analyzing alternative splicing (AS) data. The model accurately identifies condition-specific splicing changes, revealing novel tissue-specific splicing signals.

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Last Updated: Jun 12, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Alternative splicing (AS) impacts transcriptome complexity and is implicated in diseases.
  • Accurate identification of condition-specific splicing changes is crucial for understanding regulatory mechanisms.
  • High-throughput technologies allow simultaneous profiling of thousands of exons.

Purpose of the Study:

  • To develop a probabilistic model for analyzing high-throughput alternative splicing data.
  • To accurately detect common splicing signals and identify relevant exons across conditions.
  • To incorporate prior knowledge, measurement quality, and gene expression effects into AS analysis.

Main Methods:

  • Developed a probabilistic model to explain observed isoform levels as combinations of condition-specific AS signals.
  • Formulated tasks to detect common signals and identify relevant exons from AS datasets.
  • Incorporated prior knowledge, measurement quality, and gene expression level effects.

Main Results:

  • Demonstrated the advantage of the proposed method over standard approaches using a large-scale multi-tissue AS dataset.
  • Identified novel tissue-specific alternative splicing signals.
  • Experimentally verified newly found splicing signals and discussed associated regulatory features.

Conclusions:

  • The developed probabilistic model provides an accurate method for analyzing high-throughput alternative splicing data.
  • The model facilitates the discovery of condition-specific splicing events and their regulatory elements.
  • This approach enhances the understanding of alternative splicing's role in biological processes and diseases.