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Gene expression analysis by cDNA microarray in oral cancers from two Western populations.
Mai Lill Suhr Lunde1, Saman Warnakulasuriya, Lars Sand
1Department of Biomedicine, Jonas Lies vei 91, 5009 Bergen, Norway. mai.suhr@biomed.uib.no
Anticancer Research
|June 10, 2010
Summary
Gene expression profiling in oral cancer (OC) from Swedish and UK patients revealed 42 differentially expressed genes. While distinct population patterns emerged, no direct link to clinicopathological data was found, suggesting conserved tumor development pathways.
Area of Science:
- Genomics
- Oncology
- Molecular Biology
Background:
- Investigated gene expression profiles in oral cancer (OC) patients from Sweden (n=8) and UK (n=11).
- Examined the correlation between gene expression and clinicopathological data.
Purpose of the Study:
- To identify differentially expressed genes in oral cancer.
- To determine if gene expression patterns correlate with patient origin or clinicopathological parameters.
- To validate findings using quantitative reverse transcriptase polymerase chain reaction (Q-RT-PCR).
Main Methods:
- Utilized 21k human oligonucleotide microarrays for gene expression analysis.
- Extracted total RNA, synthesized cDNA, and labeled with fluorescent dyes.
- Analyzed data using Genepix and J-Express; validated results with Q-RT-PCR.
Main Results:
- Identified 42 differentially expressed genes, including 39 with known functions (e.g., FASN, NT5E, HMGA1, FOS) and 3 novel genes.
- 67% of known genes were previously reported in oral/head and neck tumors from other populations.
- Hierarchical clustering showed samples primarily grouped by population, not clinicopathological features.
Conclusions:
- Microarrays can reveal distinct gene expression patterns across different populations in oral cancer.
- No direct association was found between identified gene expression patterns and clinicopathological parameters.
- High concordance of known genes suggests conserved biological pathways in oral tumor development regardless of patient origin.
