Related Experiment Video
Updated: Jun 12, 2026

Using the Open-Source MALDI TOF-MS IDBac Pipeline for Analysis of Microbial Protein and Specialized Metabolite Data
Published on: May 15, 2019
Introduction of new tools for chemical biology research on microbial metabolites
1Chemical Biology Department, RIKEN Advanced Science Institute, Saitama, Japan. hisyo@riken.jp
Abstract:
Recently, high-throughput screening (HTS) has become the mainstream technique for drug discovery. Compounds that are synthesized by combinatorial chemistry might be more suitable than natural products to apply to HTS, because the purification procedure is a drawback of using natural products. Nevertheless, natural products remain an extremely important source of drugs. To overcome the demerits of natural products, we are constructing the RIKEN Natural Products Depository (NPDepo) that is focused primarily on microbial metabolites. In this review, I describe (i) engineering pathways for biosynthetic gene clusters of microbial metabolites, (ii) construction of fraction libraries of microbial metabolites, and (iii) the development of a new screening system using a chemical array and a protein library produced by GLORIA.
More Related Videos
07:11CorrelationCalculator and Filigree: Tools for Data-Driven Network Analysis of Metabolomics Data
Published on: November 10, 2023
14:42Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
Published on: September 23, 2021
Related Concept Videos
Microbial Biosensors
MALDI-TOF Mass Spectrometry
Methods to Assess Microbial Communities
Methods to Assess Microbial Populations