Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Guillain-Barré syndrome in a patient with antibodies against SARS-COV-2.

European journal of neurology·2020
Same author

Early thrombosis prophylaxis with enoxaparin is not associated with hematoma expansion in patients with spontaneous intracerebral hemorrhage.

European journal of neurology·2018
Same author

Intracranial pressure thresholds in severe traumatic brain injury: Con : The injured brain is not aware of ICP thresholds!

Intensive care medicine·2018
Same author

Erratum to: Acyclovir resistance in herpes simplex virus type I encephalitis: a case report.

Journal of neurovirology·2017
Same author

Behavioral characterization of the anterior injection model of subarachnoid hemorrhage.

Behavioural brain research·2017
Same author

Acyclovir resistance in herpes simplex virus type I encephalitis: a case report.

Journal of neurovirology·2016
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2026

ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis
07:11

ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis

Published on: August 19, 2021

Analysis of spectra using correlation functions.

R Beer, R H Norton

    Applied Optics
    |June 10, 2010
    PubMed
    Summary
    This summary is machine-generated.

    A new spectral analysis method uses cross-correlation and a novel heteromorphic coefficient (H) for precise, noise-immune quantitative analysis. This technique enables the detection of weak spectra and accurate Doppler shift and radial velocity measurements.

    More Related Videos

    Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
    06:51

    Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

    Published on: August 2, 2018

    O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression
    06:50

    O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression

    Published on: November 8, 2019

    Related Experiment Videos

    Last Updated: Jun 12, 2026

    ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis
    07:11

    ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis

    Published on: August 19, 2021

    Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
    06:51

    Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

    Published on: August 2, 2018

    O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression
    06:50

    O-cresol Concentration Online Measurement Based On Near Infrared Spectroscopy Via Partial Least Square Regression

    Published on: November 8, 2019

    Area of Science:

    • Spectroscopy
    • Astrophysics
    • Atmospheric Science

    Background:

    • Quantitative spectral analysis is crucial for understanding molecular composition and physical conditions.
    • Existing methods can be limited by noise and require significant manual intervention.

    Purpose of the Study:

    • To introduce a novel, robust method for quantitative spectral analysis.
    • To develop a new goodness-of-fit criterion for automated spectral analysis.
    • To enable the analysis of weak spectral data and extract precise kinematic information.

    Main Methods:

    • Utilizing cross-correlation between real spectra and synthesized or simulated spectra.
    • Introducing the heteromorphic coefficient (H) as a goodness-of-fit criterion.
    • Demonstrating noise-immunity and precision in Doppler shift and radial velocity measurements.

    Main Results:

    • The heteromorphic coefficient (H) provides a smooth, zero-crossing indicator of spectral fit.
    • The method is robust against noise, allowing analysis of spectra below the apparent noise level.
    • High precision in determining Doppler shift and radial velocity was achieved.

    Conclusions:

    • The novel spectral analysis method offers a powerful tool for quantitative analysis, particularly for weak spectral data.
    • The heteromorphic coefficient (H) facilitates automatic or near-automatic spectral fitting and analysis.
    • The technique has been successfully applied in the Atmospheric Trace Molecule Spectroscopy (ATMOS) project.