Related Experiment Videos
Human enteric adenovirus type 41 binding to HEp-2 cells is specific
1Department of Microbiology, Immunology, Louisiana State University Medical Center, New Orleans 70112-1393.
Virology
|July 1, 1991
Summary
Human enteric adenovirus type 41 (Ad41) specifically binds to a single type of protein receptor on HEp-2 cells. This interaction is temperature-dependent, saturable, and characterized by specific binding kinetics.
Area of Science:
- Virology
- Cell Biology
- Molecular Interactions
Background:
- Human enteric adenovirus type 41 (Ad41) is a significant cause of gastroenteritis in children.
- Understanding virus-receptor interactions is crucial for developing antiviral strategies.
Purpose of the Study:
- To characterize the binding properties of Ad41 to its receptor on HEp-2 cells.
- To determine the nature of the Ad41 receptor on the cell surface.
Main Methods:
- Investigated Ad41 binding to HEp-2 cells under varying temperatures.
- Analyzed binding data to determine receptor site number and affinity.
- Utilized enzymatic treatments (trypsin) and inhibitors (tunicamycin, 2-deoxyglucose, cycloheximide) to probe receptor composition.
Main Results:
- Ad41 binding is temperature-dependent, saturable, and specific.
- Identified approximately 4.3 x 10^4 receptor sites per cell with an equilibrium dissociation constant of 21.0 nM.
- HEp-2 cells treated with tunicamycin or 2-deoxyglucose recovered Ad41 binding, suggesting a proteinaceous receptor.
Conclusions:
- A single class of protein receptor mediates Ad41 binding to HEp-2 cells.
- The specific binding characteristics provide insights into Ad41's cellular entry mechanism.