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Related Experiment Video

Updated: Jun 12, 2026

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

Published on: June 28, 2019

Bypassing cellular senescence by genetic screening tools.

Mar Vergel¹, Amancio Carnero

¹Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío, Sevilla, Spain.

Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico

|June 11, 2010

Summary

This summary is machine-generated.

Bypassing cellular senescence is crucial for tumor development. Identifying genes that regulate senescence and immortalization offers new targets for cancer prevention and treatment.

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Last Updated: Jun 12, 2026

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

SA-β-Galactosidase-Based Screening Assay for the Identification of Senotherapeutic Drugs

Published on: June 28, 2019

Induction and Validation of Cellular Senescence in Primary Human Cells

Induction and Validation of Cellular Senescence in Primary Human Cells

Published on: June 20, 2018

Techniques to Induce and Quantify Cellular Senescence

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

Area of Science:

Cell Biology
Cancer Research
Genetics

Background:

Cellular senescence is a barrier to tumorigenesis.
Loss of tumor suppressor genes like p53 or pRB is insufficient for immortalization, implying additional genetic or epigenetic alterations are required.
Early research relied on labor-intensive somatic-cell genetic studies.

Purpose of the Study:

To identify genes regulating cellular senescence and immortalization.
To explore the molecular mechanisms underlying senescence regulation.
To establish the role of genetic alterations in tumorigenesis.

Main Methods:

Retroviral-based functional genetic screening.
In vivo models.
Somatic-cell genetic studies.

Main Results:

Identified universal genes that regulate senescence/immortalization through gain or loss of function.
Found that some of these identified genes are frequently altered in human tumors.
Established the causal link between genetic alterations in these genes and tumorigenesis.

Conclusions:

Genes and pathways controlling senescence/immortalization are critical in cancer development.
These identified genes and pathways represent potential novel molecular targets for cancer therapy and prevention.