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Updated: Jun 12, 2026
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Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
Published on: May 10, 2022
Systemically dispersed innate IL-13-expressing cells in type 2 immunity
April E Price1, Hong-Erh Liang, Brandon M Sullivan
1Howard Hughes Medical Institute, Department of Medicine, University of California, San Francisco, CA 94143-0795, USA.
Summary
Innate immune cells, termed Ih2 cells, secrete key cytokines like IL-13 to combat parasitic helminths. These cells are crucial for initiating type 2 immunity, even without adaptive immune system involvement.
Area of Science:
- Immunology
- Innate Immunity
- Type 2 Immunity
Background:
- Type 2 immunity relies on cytokines IL-4 and IL-13.
- Innate cells contribute to adaptive immunity initiation.
- A novel lineage-negative cell population secretes IL-13 and IL-5 in response to IL-25 and IL-33.
Purpose of the Study:
- To track lineage-negative innate cells involved in type 2 immunity.
- To investigate the role of IL-25 and IL-33 responsive cells in vivo.
- To characterize a novel innate cell population contributing to type 2 immune responses.
Main Methods:
- Utilized IL-4 and IL-13 reporter mice.
- Tracked lineage-negative innate cells during type 2 immunity and in response to IL-25/IL-33.
- Administered exogenous IL-25/IL-33 and infected mice with Nippostrongylus brasiliensis.
Main Results:
- Lineage-negative, IL-25/IL-33 responsive cells are widely distributed in mouse tissues, especially lymphoid organs and liver.
- These cells expand robustly upon IL-25/IL-33 stimulation and helminth infection.
- They are the primary innate source of IL-13 during type 2 immunity.
- IL-25 activation of these cells promotes worm clearance independently of adaptive immunity.
Conclusions:
- Identified and designated a novel innate cell population as Ih2 cells.
- Ih2 cells are integral to type 2 immune responses.
- These cells play a significant role in host defense against parasitic helminths.
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