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The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

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A novel function for the Rab5 effector Rabenosyn-5 in planar cell polarity.

Giovanna Mottola¹, Anne-Kathrin Classen, Marcos González-Gaitán

¹Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.

Development (Cambridge, England)

|June 11, 2010

Summary

Rabenosyn-5 (Rbsn-5) regulates endocytosis and planar cell polarity (PCP) in Drosophila wings. Loss of Rbsn-5 disrupts PCP protein trafficking and hair formation, highlighting its crucial role in epithelial development.

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Area of Science:

Cell Biology
Developmental Biology
Epithelial Biology

Background:

Epithelia exhibit apicobasal and planar cell polarity (PCP).
The role of polarized endocytosis in establishing PCP is not fully understood.
Rabenosyn-5 (Rbsn-5), a Rab5 effector, is involved in endocytosis.

Purpose of the Study:

Investigate the function of Rbsn-5 in Drosophila wing epithelium development.
Determine Rbsn-5's role in planar cell polarity (PCP).

Main Methods:

Studied Rbsn-5 in Drosophila wing epithelium.
Analyzed protein localization and trafficking using microscopy.
Observed effects of Rbsn-5 loss-of-function on PCP and hair formation.

Main Results:

Rbsn-5 regulates apical endocytosis in the wing epithelium.
Rbsn-5 plays a novel role in PCP establishment and maintenance.
Rbsn-5 is recruited to apical cell boundaries and redistributes along the proximodistal axis in an Fmi-dependent manner.
Loss of Rbsn-5 leads to Fmi accumulation, altered cell packing, and defects in hair orientation and formation.

Conclusions:

Rbsn-5 is essential for regulating endocytic trafficking of Fmi at apical cell boundaries.
Rbsn-5 activity is required for establishing planar polarity and proper hair morphology.
This study reveals a novel function of Rbsn-5 in planar cell polarity.