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Updated: Sep 15, 2026

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
Published on: June 26, 2020
Characteristics of histamine-induced inositol phospholipid hydrolysis in HeLa cells
1Department of Pharmacology, University of Cambridge, United Kingdom.
Abstract:
HeLa cells, a human epithelial carcinoma, contain Histamine H1-receptors, as defined by [3H] mepyramine binding studies. Histamine, via H1-receptors, stimulates the production of inositol phosphates (IP) with an EC50 of 1.2 microM. Preincubation of HeLa cells with histamine, followed by a further histamine challenge results in a slower time course of [3H] IP production, suggesting the occurrence of H1-receptor desensitisation.
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