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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...

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Related Experiment Video

Updated: Jun 12, 2026

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
09:04

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Measurement of single-cell dynamics.

David G Spiller1, Christopher D Wood, David A Rand

  • 1Centre for Cell Imaging, School of Biological Sciences, Bioscience Research Building, Crown Street, Liverpool L69 7ZB, UK.

Nature
|June 11, 2010
PubMed
Summary

Understanding cell plasticity requires measuring molecular processes in single cells. Dynamic, multiparameter methods are needed to capture complex cell-fate decisions over time.

Area of Science:

  • Cell Biology
  • Systems Biology
  • Biophysics

Background:

  • Cell populations exhibit significant heterogeneity in function and fate.
  • Understanding cell plasticity is crucial for deciphering complex biological processes.
  • Cellular processes occur across a wide range of timescales, from seconds to days.

Purpose of the Study:

  • To highlight the necessity of quantitative, dynamic measurements of molecular processes in single cells.
  • To emphasize the importance of understanding the mechanisms underlying cell-fate decisions.
  • To underscore the need for integrated experimental and computational approaches.

Main Methods:

  • Quantitative measurement of molecular processes at the single-cell level.
  • Dynamic analysis of cell signaling, intracellular processes, and transcriptional changes.

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Last Updated: Jun 12, 2026

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
09:04

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy
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Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

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Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

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  • Integration of multiparameter experimental data with mathematical simulations.

    • Main Results:

    • Early cell signaling events occur rapidly (seconds).
    • Intracellular signaling and transcriptional changes occur over minutes to hours.
    • Cell-fate decisions manifest over extended periods (hours to days).

    Conclusions:

    • Highly dynamic and complex mechanisms control cell plasticity and fate.
    • Multiparameter methods are essential for studying these dynamic processes.
    • Integrated quantitative measurement and mathematical modeling are required to understand cell fate.