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Updated: Jun 12, 2026

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines
Published on: November 8, 2011
Epstein-Barr virus lytic cycle activation alters proteasome subunit expression in Burkitt's lymphoma cells
Alessandra De Leo1, Giulia Matusali, Giuseppe Arena
1Department of Public Health Sciences, University ‘La Sapienza’, P. le A. Moro 5, I-00185 Rome, Italy.
Abstract:
We have shown that Epstein-Barr virus (EBV) lytic cycle activation in Burkitt's lymphoma (BL) cells down-regulates chymotrypsin- and caspase-like activities of the proteasome. The aim of the present study was to evaluate whether EBV activation might also affect proteasome subunit composition. Our results indicate that, independently of the latency program established in the host cells, induction of the EBV lytic cycle reduces the expression of the proteasomal components β5, β1 and β2i, whereas it increases that of β2, β1i, PA28α and PA28β. The modulation of the composition and enzymatic activities of the proteolytic complex are indicative of a less efficient generation of viral immunoepitopes.
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