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Synthesis and Assay of Vibrio Quorum Sensing Inhibitors
Published on: May 31, 2024
Desulfovibrio spp. survive within KB cells and modulate inflammatory responses
C Bisson-Boutelliez1, F Massin, D Dumas
1Laboratoire de Bactériologie-UMR CNRS 7565, Faculté de Médecine, Nancy Université, Nancy, France.
Molecular Oral Microbiology
|June 12, 2010
Summary
Certain Desulfovibrio bacteria can invade oral epithelial cells and trigger immune responses. This survival and immune modulation by Desulfovibrio species may contribute to periodontal disease development.
Area of Science:
- Microbiology
- Oral Biology
- Immunology
Background:
- Desulfovibrio are sulfate-reducing anaerobic bacteria.
- They have been implicated as potential periodontopathogens.
Purpose of the Study:
- To investigate the capacity of Desulfovibrio to invade epithelial cells.
- To determine if Desulfovibrio can induce cytokine secretion from these cells.
Main Methods:
- Co-culture of Desulfovibrio strains with KB epithelial cells.
- Evaluation of intracellular bacterial counts up to 3 days post-infection.
- Confirmation of intracellular location using confocal laser scanning microscopy and transmission electron microscopy.
- Assessment of invasion mechanism via nocodazole and cytochalasin D treatment.
- Measurement of IL-6 and IL-8 production by infected KB cells.
Main Results:
- Desulfovibrio desulfuricans and Desulfovibrio fairfieldensis survived within KB epithelial cells.
- Intracytoplasmic location was confirmed by microscopy.
- Bacterial internalization involved microtubule rearrangements, as indicated by nocodazole sensitivity.
- Infection led to increased IL-6 and IL-8 production by epithelial cells.
Conclusions:
- Desulfovibrio species can invade and survive within oral epithelial cells.
- These bacteria modulate the epithelial immune response by inducing cytokine secretion.
- The findings suggest a role for Desulfovibrio in the initiation and progression of periodontal diseases.
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