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Competitive Genomic Screens of Barcoded Yeast Libraries
Published on: August 11, 2011
Global fitness profiling of fission yeast deletion strains by barcode sequencing
Tian Xu Han1, Xing-Ya Xu, Mei-Jun Zhang
1National Institute of Biological Sciences, 7 Science Park Road, Zhongguancun Life Science Park, Beijing, 102206, PR China.
Genome Biology
|June 12, 2010
Abstract:
A genome-wide deletion library is a powerful tool for probing gene functions and one has recently become available for the fission yeast Schizosaccharomyces pombe. Here we use deep sequencing to accurately characterize the barcode sequences in the deletion library, thus enabling the quantitative measurement of the fitness of fission yeast deletion strains by barcode sequencing.

