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Updated: Jun 12, 2026

Loop-mediated Isothermal Amplification (LAMP) Assays for the Species-specific Detection of Eimeria that Infect Chickens
Published on: February 20, 2015
Detection of enteroaggregative Escherichia coli by loop-mediated isothermal amplification
Eiji Yokoyama1, Masako Uchimura, Kenitiro Ito
1Division of Bacteriology, Chiba Prefectural Institute of Public Health, 666-2, Chuo, Chiba City, Chiba 260-8715, Japan. e.ykym@ma.pref.chiba.lg.jp
Abstract:
A novel gene amplification method, loop-mediated isothermal amplification (LAMP), has been recently developed as a rapid, specific diagnostic method for various infectious diseases. We have investigated whether LAMP can be used to detect small numbers of enteroaggregative Escherichia coli (EAEC) cells contaminated in food samples. Primers for LAMP reaction were designed with EAEC aggR gene sequences (available in GenBank). LAMP specificity with these primers was the same as that of PCR in a study of 37 EAEC and 42 non-EAEC bacterial strains. The sensitivity of the LAMP method was better than that of PCR in a study of serially diluted EAEC cells. The LAMP method was significantly more effective than was PCR in detecting EAEC-contaminated food samples (Fisher's exact test, P < 0.05). Therefore, the LAMP method described here should be useful for detecting small numbers of EAEC cells in food samples.
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