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Published on: September 20, 2021
GPR155: Gene organization, multiple mRNA splice variants and expression in mouse central nervous system
Stefan Trifonov1, Takeshi Houtani, Jun-Ichi Shimizu
1Department of Anatomy and Brain Science, Kansai Medical University, Moriguchi, Osaka 570-8506, Japan.
Biochemical and Biophysical Research Communications
|June 12, 2010
Summary
This study details the G protein-coupled receptor 155 (GPR155) gene structure and its mRNA variants in mice. GPR155 is widely expressed in mouse tissues and brain regions, with potential roles in neurological disorders.
Area of Science:
- Neuroscience
- Genetics
- Molecular Biology
Background:
- G protein-coupled receptor 155 (GPR155) is implicated in Huntington's disease and autism spectrum disorders.
- Understanding GPR155's genetic structure and expression is crucial for neurological research.
Purpose of the Study:
- To elucidate the structural organization of the mouse GPR155 gene.
- To identify and characterize GPR155 mRNA variants and their expression patterns in mouse tissues and brain.
- To predict the protein secondary structure and functional domains of GPR155.
Main Methods:
- Gene structure analysis and mRNA variant generation.
- Quantitative analysis of GPR155 mRNA expression in adult and developing mouse tissues.
- In situ hybridization to map GPR155 mRNA distribution in the mouse brain.
- Bioinformatic prediction of protein secondary structure and domains.
Main Results:
- Five GPR155 mRNA variants (Variants 1-5) were identified, including four novel variants.
- GPR155 mRNA is broadly expressed across adult mouse tissues and during development.
- GPR155 mRNA shows widespread distribution in forebrain regions, with concentrated expression in the lateral striatum and hippocampus.
- Expression patterns in the striatum resemble those of cannabinoid receptor type 1.
- GPR155 is predicted to be a 17-transmembrane protein, with Variants 1 and 5 possessing a conserved intracellular DEP domain.
Conclusions:
- The study provides a comprehensive analysis of the mouse GPR155 gene, revealing novel mRNA variants and detailed expression profiles.
- The widespread expression and specific localization of GPR155 in the brain suggest significant roles in neural function and potentially in neurological diseases.
- The identification of a conserved DEP domain offers insights into GPR155's molecular function and signaling pathways.
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