Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Autoregulation of Blood Flow01:17

Autoregulation of Blood Flow

Autoregulation mechanisms are characterized by their inherent capacity for self-regulation without necessitating specific nervous stimulation or endocrine control. These mechanisms facilitate the adjustment of blood flow and, therefore, perfusion specific to each tissue region. This self-regulation encompasses chemical signals and myogenic controls.
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Outcomes of ear endoscopic surgery for petrous bone cholesteatoma].

Zhonghua yi xue za zhi·2025
Same author

Molecular characterisation and expression profile of the <i>PRLR</i> gene during goose ovarian follicle development.

British poultry science·2023
Same author

A 2-year locomotive exploration and scientific investigation of the lunar farside by the Yutu-2 rover.

Science robotics·2022
Same author

Crisaborole: an emerging therapy for prurigo pigmentosa.

Journal of the European Academy of Dermatology and Venereology : JEADV·2022
Same author

Golgi phosphoprotein-3 (GOLPH3) promote metastasis of nasopharyngeal carcinoma through regulating E-cadherin.

European review for medical and pharmacological sciences·2020
Same author

Morroniside suppresses hydrogen peroxide-stimulated autophagy and apoptosis in rat ovarian granulosa cells through the PI3K/AKT/mTOR pathway.

Human & experimental toxicology·2020

Related Experiment Video

Updated: Jun 12, 2026

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies
09:03

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Published on: June 30, 2023

Controlling physiological angiogenesis by hypoxia-induced signaling.

E Hadjipanayi1, R A Brown, V Mudera

  • 1UCL Division of Surgery and Interventional Sciences, Tissue Repair and Engineering Centre, Brockley Hill, Stanmore Campus, London HA74LP, UK.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|June 12, 2010
PubMed
Summary

Engineered Hypoxia-Induced Signaling (HIS) cells in a 3D model promote targeted blood vessel growth. This breakthrough advances tissue engineering and therapeutic angiogenesis by precisely controlling local hypoxia for predictable vascularization.

More Related Videos

Induction and Testing of Hypoxia in Cell Culture
07:01

Induction and Testing of Hypoxia in Cell Culture

Published on: August 12, 2011

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Related Experiment Videos

Last Updated: Jun 12, 2026

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies
09:03

Modified In Vivo Matrix Gel Plug Assay for Angiogenesis Studies

Published on: June 30, 2023

Induction and Testing of Hypoxia in Cell Culture
07:01

Induction and Testing of Hypoxia in Cell Culture

Published on: August 12, 2011

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Vascular Biology

Background:

  • Angiogenesis, the formation of new blood vessels, is crucial for tissue repair and development.
  • Controlled angiogenesis is essential for therapeutic applications but challenging to achieve precisely.
  • Tissue hypoxia is a known trigger for angiogenic factor cascades.

Purpose of the Study:

  • To develop and validate a 3D in vitro model for testing localized hypoxic stimuli.
  • To engineer a cell-based system for controlled induction of angiogenesis.
  • To assess the efficacy of engineered Hypoxia-Induced Signaling (HIS) cells in promoting vascularization.

Main Methods:

  • Designed a 3D collagen matrix model with spatially positioned cell depots.
  • Engineered HIS cells from human dermal fibroblasts (HDFs) to create localized hypoxia.
  • Co-cultured HIS cells with endothelial cells (ECs) to observe angiogenic responses.
  • Implanted constructs in vivo and monitored vascularization and oxygen levels.

Main Results:

  • HIS cell depots released angiogenic factors, inducing directional EC migration and tubule formation.
  • Non-hypoxic controls showed minimal EC migration and tubule formation.
  • HIS cell depots significantly accelerated host vessel in-growth into 3D constructs by at least 7 days.
  • In vivo vascularization functionality was confirmed by real-time oxygen monitoring.

Conclusions:

  • HIS cells effectively induce localized angiogenesis in a 3D model.
  • The developed model allows for predictable control over vascularization.
  • This approach holds promise for in vitro tissue modeling, implant vascularization, and therapeutic angiogenesis strategies.