[Differences in IgG and IgE antibody responses to a low-molecular compound in inbred strains of mice]

It appears to be significant for the analytical studies of drug allergy that a comparison is made among inbred strains of mice, with different H-2 haplotypes with respect to the antibody response to low-molecular compounds. Such studies have been demonstrated by several investigators using high-molecular antigens, such as the synthetic polypeptides and natural proteins. The present experiments were undertaken, therefore, to clarify the differences in antibody responses to a low-molecular compound, TNBS in inbred strains of mice. Mice of four different strains, A/J (H-2a), C57BL/6 (H-2b), DBA/2 (H-2d) and C3H/He (H-2k) were immunized with TNBS (30 & 300 micrograms/body) plus FCA by the routes of ip or sc. Responses of IgG and IgE antibody productions were estimated by HA in serum samples treated with 2-ME and rat PCA reaction in untreated serum samples, respectively. As the results, DBA/2 (H-2d) mice showed high responses on IgE antibody production whereas C3H/He (H-2k) mice on IgG antibody production. A/J (H-2a) mice displayed high responses both on IgG and IgE antibody productions. C57BL/6 (H-2b) mice denoted low to intermediate responses both on IgG and IgE antibody productions. The above phenomenon may be interpreted by the fact that H-2a haplotype of A/J mice has a crossover on K region-E alpha subregion of H-2k and S.D regions of H-2d.